Careers. 0000019743 00000 n Notice how the chance of a live birth is predicted to decrease with age (discussed more below), and that day 3 embryos with fewer cells generally dont do as well. 2005). PGS testing was done before initial freeze. (2022) created models to predict live birth rates based on 223,377 embryo transfers in the US. trailer Why did my embryo grade change after it was thawed? Up to 20 percent fragmentation is fine. Before reading this please be aware that these success rates are based on large groups of women who were all treated in similar ways, at the same clinic (usually), with similar backgrounds (baseline characteristics, such as age, BMI, diagnosis, etc.). A single euploid embryo was selected for transfer per cycle on either the morning of d6, for fresh transfers or 5 days after progesterone supplementation for patients with transfer in an FET cycle. Its kind of like the way youd make a good snowball. The Prelude Connect App is now available for AFCC patients! Results: Of 676 euploid embryos included in this analysis, 464 were expanded or hatching (Ex/HgBl) and 212 were completely hatched (CHBl) at the time of transfer. Some clinics will discard these embryos, and its important to check with your clinic what their policy is on this. Eggs can be fertilized by conventional IVF or by intracytoplasmic sperm injection (ICSI). My fully hatched blasts are now 17 months old. Honestly I'm not finding a lot of success with FET in general. Bookshelf Blastocysts in this analysis were all tested for aneuploidy, by NextGen Sequencing. You can learn more about how we ensure our content is accurate and current by reading our. 2001). Group Owners uphold the core values of the brand by reporting content that violates the community guidelines. PMC If you thought that grading a day 3 embryo was challenging, things get even more complicated with day 5 embryos. Some embryos will have 3, 5, or 6 cells, and thats because cells dont divide at the same time. Some evidence suggests a hatching blastocyst is more likely to split into twins although other studies have found no significant increase. This is usually done before freezing to remove the water from inside the embryo. We respect everyones right to express their thoughts and opinions as long as they remain respectful of other community members, and meet What to Expects Terms of Use. I have a friend who has identical pgd boys from her first pgd embryo that was also fully hatched. 0 You can actually tell fertilization is occurring because you can see the nucleus of the egg and sperm cell (called the pronuclei, as plural). Since the zona is gone, some worry that these embryos are more exposed and more fragile. (2014) looked at Day 3 double embryo transfers (average age of 30). So in my case it was good. In this time, the embryo cant yet use its genome to make the proteins it needs to divide and grow, so it uses stored factors that were left behind in the egg to develop. There are no conflicts of interest to declare.. Keywords: Whats an A graded trophectoderm to one embryologist, may be a B to another (even from the same clinic). Been struggling with my babe only taking contact naps so Ive really been trying to get him to sleep in his bassinet on his own.Today I followed his sleepy cues very carefully and brought his bassinet (that he sleeps in at night) into his nursery My LO will be 3mo on the 21st and weve had a love/hate relationship with swaddles ever since he turned about 3 weeks old. The first step of the IVF process is stimulating your ovaries to produce multiple mature eggs, using fertility medication. Now in the 2ww so hping for a great outcome. Awadalla et al. Once fertilization has occurred, the ZP hardens to prevent additional sperm from penetrating the egg and prevents premature implantation in the fallopian tube (ectopic pregnancy). As it grows in size, the embryo stretches and thins out the zona. Trophectoderm (TE) score, or quality - range A - C (A being the best) So the least advanced blastocyst would be 1CC, and the most would be 6AA. Wondering if this could be possible twin pregnancy? Clipboard, Search History, and several other advanced features are temporarily unavailable. Would love to hear what others have experienced when transferring a fully hatched embryo. All rights reserved Advanced Fertility Center of Chicago, Female Age and Chromosome Problems in Eggs and Embryos, Antral Follicle Counts, Resting Follicles and Ovarian Reserve, Money back guarantee plans for frozen donor eggs, Advanced Fertility Center of ChicagoChicago, 4920 N Central Ave #2C, Chicago, IL 60630, Advanced Fertility Center of ChicagoCrystal Lake, 820 E Terra Cotta Ave, Crystal Lake, IL 60014, Advanced Fertility Center of ChicagoGurnee, Advanced Fertility Center of ChicagoDowners Grove, 3800 Highland Avenue Suite 110 Downers Grove, IL 60515, 5 day blastocyst transfer success rates are potentially confusing. Studies of blastocyst collapse in different species of mammals have found that most blastocysts, that experience consecutive weak contractions, hatch successfully whereas those that exhibit strong contractions or collapse, fail to hatch. For many women afflicted with infertility, this step is compromised. Need help understanding your day 5 (blastocyst) embryo grade and picture? They found that day 3 embryos with more than 10 cells had comparable implantation, pregnancy, live birth and multiple pregnancy rates compared to 8 cell embryos. 0000014143 00000 n Both implanted ans currently almost 10 weeks with twins. In the video Ill also spend some time going over the parts of an embryo, embryo development and how grading works, using plenty of pictures to make sense of it all. IVF Success Rates with 5 Day Blastocyst Transfers at the Advanced Fertility Center of Chicago. Assisted hatching is, therefore, a valuable technique to use in the laboratory for PGT-A cases. These two cell types are important when it comes to blastocyst embryo grading. A Group Owner is a member that has initiated the creation of a group to connect with other members to share their journey through the same pregnancy & baby stages. Please whitelist our site to get all the best deals and offers from our partners. My first FET (chemical pregnancy) was a 5 day embryo. 0000002844 00000 n Wijnland Fertility grades embryos according to three parameters. Storr et al. Limitations include the retrospective design and data from a single institution. View their partner profile here and check out their website by clicking here. (2020)found no difference in gestational age, birth weights, complications, and other outcomes among nearly 100 births from day 7 embryos compared to day 5 or 6. *, Clinical outcomes segregated according to the time the transferred embryo hatched. 1994 . Interestingly, like most things in IVF, the answer is not clear! SHBT group showed significantly higher blastocyst formation rate (53.3 17.5 vs. 43.1 14.5%, P = 0.0098), top-quality blastocysts (71.8 vs. 53.7%, P = 0.0436), IR (43.6 vs. 27.9%, P =. Usually around day 5 or so, the blastocyst forms. I should also add that my symptoms were also negligible. BFP - No heartbeat and growth 3+wks behind at 9 wks . A Group Owner is a member that has initiated the creation of a group to connect with other members to share their journey through the same pregnancy & baby stages. doi: 10.1371/journal.pone.0267652. 2017 Mar 1;23(2):139-155. doi: 10.1093/humupd/dmw038. In some cases the expansion might change, or even the embryos quality. Performing assisted hatching on these embryos could facilitate embryo hatching and possibly improve the chances of implantation. Ill explain the way I learned when I was an embryologist. Error bars represent 95% confidence interval (CI). IVF pregnancy with fully hatched embryo. What to Expect supports Group Black and its mission to increase greater diversity in media voices and media ownership. This cavity collects water and allows for the embryo to expand as it takes in more water. (2014)looked at single embryo transfers of 849 blasts between 2008-2012 in women <40 years of age. *. At Fertility Centers of New England our laboratory scoring uses a descriptive expansion EB, B, or EXB (early blastocyst, blastocyst, expanded blastocyst) and cell composition listed as a numerical score 1-4, with a score of 1 being best. (815) 356-1818, 30 Tower Court, Suite F Gurnee, Illinois So about 10% of good prognosis women, who transfer up to 3 euploids, will still fail to achieve a live birth. Do I have this correct?? A score of 3 indicated a full blastocyst with a blastocoel completely filling the embryo, 4 indicated an expanded blastocyst with a larger blastocoel volume, 5 indicated a hatching blastocyst with trophectoderm herniating through the zona pellucida, and 6 indicated a fully hatched blastocyst. We work hard to share our most timely and active conversations with you. Results: Of 676 euploid embryos included in this analysis, 464 were expanded or hatching (Ex/HgBl) and 212 were completely hatched (CHBl) at the time of transfer. In 1999, pioneering fertility doctors David Gardner and William Schoolcraft introduced a blastocyst grading system that was adopted widely by IVF laboratories because of its success rate. 4 studies showed the trophectodermwas predictive. 2021 Mar 18;36(4):929-940. doi: 10.1093/humrep/deab014. Becoming a blastocyst requires a massive shift in development. Giulia et al. Live birth rate following frozen-thawed blastocyst transfer is higher with blastocysts expanded on Day 5 than on Day 6. Assisted hatching. Other studies have found a similar impact of grades on euploids, including the impact of day 5, 6 and 7 (reviewed in my post on PGT-A (PGS) success rates). Babycats. Every effort is made to provide accurate and up-to-date information. Embryologist said the embryo looked perfect. This stage is followed by blastocyst hatching and implantation. XcPNPPI xX4Xp)770,`taj`Rqhd1ikMFffL5LbV41-+5 Wider implications of the findings: According to Goto et al. (41. Predicting live birth rates, multiples based on 223,377 transfers, Grade C (poor quality) embryo success rates, Increased risk factors with blastocyst transfer vs cleavage stage, Predicting how many day 3 embryos make it to blastocyst, Transfer of day 7 embryos a viable option, Age-specific blastocyst conversion rates and other IVF outcomes in good prognosis women, Fertilization and the pronuclear stage (day 0), Cleavage stage embryo grading (day 2 or day 3 embryo grading). A nomogram is kind of like a calculator that you can use to predict something, and this studys nomogram predicts how many blastocysts will develop. Disclaimer: These pages are meant purely for informational purposes. Archived discussions are usually a bit older and not as active as other community content. Terms are highlighted every 3rd time to avoid repetition. Of these, 436 cycles entailed transfer of a NFH blastocyst (n = 123 fresh transfer, n = 313 frozen/thawed embryo transfer (FET)) and 372 cycles entailed transfer of an FH blastocyst (n = 132 fresh, 240 FET). 0000035459 00000 n embryos with more than 8 cells have comparable success rates to 8-cell embryos, Fast growing day 3 embryos and their reproductive potential. Researchers are divided whether this is a good sign or something to worry about. The skin of the balloon is made up of the trophectoderm cells that surround a cavity called the blastocoel, which is filled with water. Surrounding the blastocyst is the zona pellucida, or zona for short, which is like a shell that was originally present in the egg and carried on. The main outcome measure was IR. (847) 662-1818, 3800 Highland Avenue Suite 110 Downers Grove, IL 60515 Which is what made us decide to have our next batch PGS and PGD tested. 2. Heres another way to look at this: There can be variability in grading the expansion depending on the clinic and the subjectivity of the embryologist. TMI - I had diarrhoea at about 6dp5dt each time and think in hindsight this was my true implantation sign. Heres a quick guide that puts it all together: Here are some pictures of day 3 embryos and their grades. (2005). Clinical outcomes of not fully hatched versus fully hatched blastocysts in fresh and, Clinical outcomes segregated according to, Clinical outcomes segregated according to the time the transferred embryo hatched. Your post will be hidden and deleted by moderators. Good quality embryos included embryo grades 6AA, 6AB, 6BA, 5AA, 5AB, 5BA, 4AA, 4AB, 4BA, 3AA, 3AB and 3BA, which had a. Short Protocol. In other words, these statistics arent meant to be accurate for you personally. Copyright 2021. In the FET cycles, IR was similar between NFH and FH cycles (62.6% versus 61.7%, OR 1.0; 95% CI: 0.7-1.5). In the video Ill also spend some time going over the parts of an embryo, embryo development and how grading works, using plenty of pictures to make sense of it all. Poor quality embryos work check your clinics policy on discarding them, Blastocyst embryo grading (Day 5 grading), Variability in expansion embryo grades by clinic or embryologist, ICM and trophectoderm grading for early blastocysts, Embryo compaction may make embryo grading difficult, How blastocyst embryo grades are reported, Day 5 (blastocyst) embryo grades and pictures. You can read more about cytoplasmic strings in my post Improved live birth rates in blastocysts with cytoplasmic strings.. I think it's positive according to my clinic. These develop into the fetus. Most clinics now believe that transferring better-developed embryos - i.e. They found that if they stopped culturing at day 6 and didnt culture embryos to day 7, there would be a: If you wanted to read more about this study you can check my post Transfer of day 7 embryos a viable option. Study question: Bottom line: Its hard to know, based on grading alone, what your success will be. The babies began hatching Feb. 26 and continued through March 2, according to the aquarium, which is at UC San Diego's Scripps Institution of Oceanography. Fertilization is where the gametes (sex cells, like the egg and sperm cell) come together to form the embryo. Please specify a reason for deleting this reply from the community. Find advice, support and good company (and some stuff just for fun). Find out whether genetics play a. Reaching the end of the fertility trail with a prize to cuddle can be a physically and emotionally draining trek that covers so much uncharted territory. This is an interesting comparison because many clinics would transfer a good quality day 6 embryo, but would discard a poor quality day 5 embryo. (2022)compared the outcomes of day 5, 6 and 7 blastocysts that were tested for PGT-A (and were euploid). The other two I transferred were a day 6 morula (missed miscarried @6 weeks) and an ordinary day 5 blast (chemical). At least at my old clinic, the first number could vary based on factors besides fragmentation. Hatching may not always take place but it is a necessary step towards implantation and pregnancy. Day 3 embryos are graded 1 to 4 (or 5) depending on the lab protocol with 1 being the highest grade. Hopefully, that one will pass through PGD and PGS. However the miscarriage rate was lower when there was more than 10 cells. As an embryo develops to day 3, theres a chance the embryo will continue development, or will stop developing and arrest. Objective: To compare pregnancy outcomes between the transfer of PGT-A tested completely hatched blastocysts versus tested expanded or hatching blastocysts in frozen embryo transfers cycles. This has led to improvement in the outcome of IVF nationwide. You can see that the success rates for day 7 embryos are acceptable from the data above. 0000000016 00000 n I just had a FET today. By assisting with embryo hatching, the process of implantation is started. Find advice, support and good company (and some stuff just for fun). 2005-2023Everyday Health, Inc., a Ziff Davis company. He also said that embryos that are frozen and defrosted are very strong embryos. 0000002733 00000 n Pirtea et al. This discussion is archived and locked for posting. Heres a handy quick guide to help you put it all together: As mentioned, some clinics might use D in addition to ABC, while others may use A-F. Others may use grade I in place of A or use a + or - sign where an A+ would be a perfect grade. The rate of triplets with blast transfers at our IVF center has been about 2% of pregnancies. DOI: Glujovsky D, et al. (2018) found live birth rates from day 7 embryos were about half of day 5 and day 6 (~25% vs ~45%) with no differences in low birth weight, malformations or early neonatal death. 54 73 Heres an example of a cleavage stage embryo with around 8 cells: Usually around day 4 the cells undergo compaction to form a morula. I equate this to a kind of cocoon where the cells of the embryo start to change, or differentiate, into the cells of the blastocyst, which is a very different structure compared to the cleavage stage embryo. Unauthorized use of these marks is strictly prohibited. Craciunas et al. We thought it was better to let those slower embryos develop and expand more in the culture dish before choosing the best 1 or 2 for transfer. Note that once you confirm, this action cannot be undone. 0000008952 00000 n They didnt find any differences in success rates (implantation rate, pregnancy, miscarriages). 0000005244 00000 n The day of an embryo isnt really part of the grade, but its considered as research has shown that how fast an embryo grows relates to its chance of implanting. Which is comparatively high to the alternative freezing method called slow freeze with an 86% survival rate. %PDF-1.7 % Before (2020) found that good prognosis women who transferred up to 3 euploid embryos (one at a time, for a total of three transfers) had a cumulative live birth rates of 92.6% by the third transfer. Accessibility To date, no other study has evaluated the reproductive competence of an FH blastocyst transfer. Purple columns show live birth success rates for day 3 transfers 2018 Mar 1;33(3):390-398. doi: 10.1093/humrep/dey004. We have done blastocyst culture and transfer since 1998. In conventional IVF, sperm are mixed with the eggs and left to fertilize. But these embryos are considered after high quality embryos, because theyre less likely to work and doctors hope to get you pregnant as fast as possible! Cytoplasmic strings are string-like structures that can be seen in an embryo photo. 0000080636 00000 n When an embryologist grades the blastocyst from 0 to 6, it states its size. The educational health content on What To Expect is reviewed by our medical review board and team of experts to be up-to-date and in line with the latest evidence-based medical information and accepted health guidelines, including the medically reviewed What to Expect books by Heidi Murkoff. Hum Reprod. As fragments are lost, the cell that fragmented gets smaller. 0000005015 00000 n We found no evidence of altered IR or other clinical outcomes in the transfer of FH euploid embryos. (The size of the cake doesnt change when you slice it, but the number of pieces does.). Good quality day 3 embryos tend to have a higher chance of making it to blastocyst. On Day 5 there were three CCs and one BB. The number of cells is important for cleavage stage embryo grading, as well see. So tuck away all of this information in a corner of your mind and go shopping for those snugglies. Day 7 embryos work check your clinics policy on discarding them. The month leading up to an IVF cycle is critical for preparing. 0000009313 00000 n Fresh and FET cycles and associated clinical outcomes were considered separately. Exp462. And lets not forget about day 2 embryos! and transmitted securely. Finally, the conditions and protocols used in the IVF laboratory can also impact the euploid blastocyst transfer success rates. Group Leaders arent expected to spend any additional time in the community, and are not held to a set schedule. First round was bfn and now waiting to see how this second round goes - beta is Friday!! Posted 6/26/14. (2022) created models to predict live birth rates based on 223,377 embryo transfers in the US. The blastocyst has a structure called a blastocoel which is the space inside the blastocyst that fills up with water. Did you perform PGS testing with the PGD? Embryo grading and success rates is a huge topic, as it will cover an embryos development, why embryos are graded, cleavage stage (day 3) embryo grading, blastocyst stage (day 5) grading, and look at different examples of these grades with wonderful pictures that have been donated to me by my supporters! Good? Good luck! 0000004688 00000 n 0000005130 00000 n After adjusting for age, BMI, endometrial thickness at the LH surge and oocytes retrieved in an LR model, the hatching status was not shown to be associated with implantation (P > 0.05). This could change the grade that an embryo receives. Good quality (3BB) and poor quality (<3BB) were evaluated: Awadalla et al. 2 = small cavity filling a third of the embryo. 0000015984 00000 n You can check it out in my post Predicting how many day 3 embryos make it to blastocyst. I know they perform assisted hatching on all the embryos before freezing them at the clinic I use. think twice before sharing personal details, foster a friendly and supportive environment, remove fake accounts, spam and misinformation, delete posts that violate our community guidelines, reviewed by our medical review board and team of experts. Is there an optimal window of time for transferring single frozen-thawed euploid blastocysts? government site. 0000017828 00000 n (Other techniques include chemical thinning, mechanical disruption with a microneedle and piezoelectric pulses.) Fertility treatments take a toll on nonpregnant partners, too. Compaction are the cells compacted and readying for the next stage, which is forming a blastocyst? The views expressed in community are solely the opinions of participants, and do not reflect those of What to Expect. Hi, . Oron et al. 0000034981 00000 n This is good as a general guide, but newer research is showing that embryos with more than 8 cells have comparable success rates to 8-cell embryos. So, as an example, you may have a day 5 embryo thats graded as 5AA. 0000003862 00000 n In the second study there was a reduction in success rates, so this suggests the hatched embryo is more fragile. Group Leaders arent expected to spend any additional time in the community, and are not held to a set schedule. A 4AA would be represented as 4-3-3, a 4CC as 4-1-1. Disclaimer. In vitro fertilization (IVF) is a type of assistive reproductive technology (ART) that involves retrieving eggs from a womans ovaries, fertilizing. This site needs JavaScript to work properly. The usual way is by using the Gardner system, as I explained above. Please whitelist our site to get all the best deals and offers from our partners. These triplets have almost always been due to identical twin splitting of an embryo, 2 transferred and 3 implanted, after one of the embryos has split into 2. 1994. 0000003407 00000 n The ICM actually becomes the fetus, while the trophectoderm becomes the placenta! Many clinics will stop culturing embryos at day 6, and its important for you to make sure that your clinic will culture to day 7, to give you the best shot at getting as many embryos as you can. I just had a FET today. So in a 1/4 theres 4 cells. Good quality blasts (3BB) a live birth rate of 41% was achieved, 4.4% reduction in patients with a live birth, Aneuploidy (not having the right number of chromosomes), Problems with mitochondria (the batteries of the cell), Unfavorable lab conditions (ie. IVF laboratories provide numerous add-on treatments to improve IVF outcomes and the possibility of taking home a baby. Need help understanding your day 3 embryo grade and picture? This sometimes occurs when cells divide. These two cell types are important when it comes to blastocyst embryo grading. Thanks everyone! An embryo with a C grade ICM may be still developing. (2022) study shown earlier: (I have the full summary of this study on my post Predicting live birth rates, multiples based on 223,377 transfers). I had a fully hatched blast transferred yesterday. The best way to get accurate statistics will be from your clinic. High quality day 5 blastocyst stage IVF embryoBlastocyst grading. DOI: Increased success and safety in assisted reproductive technology by using elective single embryo transfer. I do know that my embryos were tested for the translocation and chromosome errors in general though as a few random errors were found in some embryos each time. 2022 Nov 24;37(12):2797-2807. doi: 10.1093/humrep/deac227. Fully hatched is great! VerMilyea. 0000004311 00000 n In my opinion these are the strongest embryo to get! ( A ) Main analysis; ( B ). For cleavage stage embryos (day 2 or 3 embryos), embryo grading is mainly based on the number of cells and the amount of fragmentation the embryo has. STUDY DESIGN, SIZE, DURATION Retrospective cohort study. For cleavage stage embryos, or day 2 or day 3 embryos, these features are typically: For morulas, these features are typically: For blastocysts, these features are typically: In addition, the number of days it took the embryo to become a specific stage is considered. Embryo grading is very subjective, and not all embryos will grade embryos the same. Its important for embryologists to receive regular training to ensure consistency, but this isnt practiced everywhere. Bad? 0000080481 00000 n I was googling for timelines after a 5dt to see when the embryo might actually implant. Additionally, the study was limited to patients that developed high-quality blastocysts suitable for biopsy. Feb 3, 2011 5:34 PM. Yes, avoiding a Robertsonian Translocation, but also couldn't get pregnant spontaneously (so have an unopened new pack of birth control I don't really care for in the bathroom taunting me). Oliva M, Briton-Jones C, Gounko D, Lee JA, Copperman AB, Sekhon L. J Assist Reprod Genet. Heads up: Blastocyst grading is complex and that means that grades arent carved in stone and may change. Good luck! In this retrospective study, the clinical value of transferring blastocysts derived from day . Thanks for sharing the successful stories. IVF outcomes in comparison to non-hatching embryos. Expansion 1 and 2 can be pretty variable because its sometimes not clear if the blastocoel is less than or more than 50%. These are cleavage stage embryos because the cells cleave, or split, to form a new cell. Each laboratory has its own grading system and embryos are usually graded by the embryologists to categorize the embryo quality. hatched blastocysts results in signicantly lower success rates when compared to other stages of blastocyst development (James, R. M et al, 2018). 2 = cavity fills 1/3 of the embryo 3 = partial expansion, fills 70% of the embryo 4 = fully expanded cavity 5 = embryo has expanded and split open the zona Improved live birth rates in blastocysts with cytoplasmic strings., Comparable outcomes with good quality day 6 and poor quality day 5 embryos, Genetic analysis of arrested embryos reveals 3 distinct types. Epub 2014 Feb 26. It was just a way to avoid embryologists from thawing the wrong embryo because a 4/4 (good quality 4 cell embryo) might be confusing to some newer people who might want to thaw and transfer that over a 2/6. Input your search keywords and press Enter. Some of the cells form the trophectoderm epithelium (TE). Check here for the full. The variable tested was the completeness or degree of the hatching status of the blastocyst at the time of transfer and the presence of fetal cardiac activity via ultrasound at 7 weeks of gestation. It has also been suggested that the complete removal of the . Because the cells can slip out easily, the zona doesnt thin out as the embryo grows. Do the reproductive outcomes from the transfer of fully hatched (FH) blastocysts differ from those of not fully hatched (NFH) blastocysts? Let's start by reviewing how blastocysts are graded: NUMBER: The number refers to the degree of expansion of the embryo's cavity and goes from 2-6. Cytoplasmic pitting has a negative influence on implantation outcome. Check it out to see a lot more! Well get into all the statistics and the studies below, so stay tuned! 126 0 obj <>stream Remember the story about the tortoise and the hare. Older women tend to produce more aneuploid eggs, which result in aneuploid embryos. Terms are highlighted every 3rd time to avoid repetition. Most embryos, however, would hatch once transferred into the uterus. Learn more about, Twins & Multiples: Your Tentative Time Table. Study funding/competing interests: He also answers questions in his private Facebook group. (2017)compared the transfer of a single early blastocyst, or expanded/hatching/hatched good, fair or poor quality embryos: Note: This study didnt indicate the number of each type of embryo for these groups.
fully hatched blastocyst success rate